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[Relationship between amplicon composition and cytologic type of structures containing amplified DNA in murine P388
Abstract:
Previously, we showed that, development of multidrug resistance (MDR) in mouse P388 leukemia cells, is often associated with the appearance of newly-formed chromosome-like structures that contain amplified copies of the mdrl gene. In the present study, we compared amplicon content in P388 sublines showing different types of these structures. A strong correlation between the formation of specific acentric markers consisting of two identical arms and the absence of the sorcin gene co-amplification was found. In all the sublines containing other types of chromosome-like structures, the sorcin gene is co-amplified.
Insights
Multidrug resistance (MDR) in leukemia cells involves gene amplification and unique chromosome structures. Specific structures correlate with the absence of sorcin gene co-amplification, while others show its co-amplification.
Area of Science:
- Genetics
- Molecular Biology
- Cancer Research
Background:
- Multidrug resistance (MDR) in mouse P388 leukemia cells is linked to amplified mdrl genes.
- Newly-formed chromosome-like structures are observed in MDR cells.
Purpose of the Study:
- To compare amplicon content in P388 sublines with different chromosome-like structures.
- To investigate the relationship between specific structures and gene co-amplification.
Main Methods:
- Analysis of amplicon content in P388 leukemia cell sublines.
- Comparison of chromosome-like structures and associated gene amplification patterns.
Main Results:
- A strong correlation was found between specific acentric markers (two identical arms) and the absence of sorcin gene co-amplification.
- All other types of chromosome-like structures were associated with sorcin gene co-amplification.
Conclusions:
- The type of chromosome-like structure in MDR P388 cells is associated with specific gene amplification patterns.
- Sorcin gene co-amplification is dependent on the morphology of the observed extrachromosomal elements.