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Staphylococcal enterotoxin is a bovine T cell superantigen
R Schmaltz1, B Bhogal, Y Y Wang
1Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, USA.
Immunological Investigations
|August 1, 1995
Summary
Staphylococcal enterotoxins (SE) activate bovine immune cells, stimulating T cell proliferation and IgG production. This suggests SE
Area of Science:
- Immunology
- Microbiology
Background:
- Staphylococcal enterotoxins (SE) are potent immune activators.
- Understanding SE's interaction with immune cells is crucial for disease pathogenesis.
Purpose of the Study:
- To investigate the effects of SE isoforms (SEB, SEC-1, SEC-2) on bovine immune cells.
- To elucidate the role of antigen-presenting cells (APCs) in SE-mediated immune responses.
Main Methods:
- Incubation of bovine peripheral blood mononuclear cells (PBMCs) with SE isoforms.
- Assessment of T cell proliferation, cytokine expression, and IgG production.
- Analysis of APC function using UV-irradiated PBMCs and anti-MHC II monoclonal antibodies (MAbs).
Main Results:
- SE isoforms stimulated bovine T cell proliferation, cytokine expression, and IgG production.
- UV-irradiated PBMCs retained the ability to present SE, indicating intact APC function is not required for presentation.
- MAbs against MHC II abolished T cell proliferation, highlighting the importance of MHC II in SE presentation.
- SE increased CD4+ and CD8+ T cells but not MHC II+ APCs or B cells.
- SE induced polyclonal IgG production in B cells without causing proliferation.
Conclusions:
- SE acts as a superantigen, activating T cells and inducing polyclonal B cell activation and IgG production.
- APCs are essential for SE presentation via MHC II molecules.
- SE's immune-activating properties may contribute to its virulence.