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Related Experiment Videos

Neomycin blood agar as a selective medium for vancomycin resistant Enterococcus faecium

P R Chadwick1, B A Oppenheim

  • 1Public Health Laboratory, Withington Hospital, Manchester.

Journal of Clinical Pathology
|November 1, 1995
PubMed
Summary

Neomycin blood agar may not effectively isolate all vancomycin-resistant enterococci due to high neomycin concentrations. Further studies are needed to optimize selective media for these emerging nosocomial pathogens.

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Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Medical Mycology

Background:

  • Vancomycin-resistant enterococci (VRE) are significant nosocomial pathogens.
  • Neomycin blood agar is a common selective medium for VRE isolation from fecal samples.
  • Suboptimal recovery of VRE on neomycin agar suggests potential issues with neomycin concentration.

Purpose of the Study:

  • To investigate the hypothesis that high neomycin concentrations in selective media limit the recovery of vancomycin-resistant Enterococcus faecium (VRE).
  • To determine the neomycin minimum inhibitory concentration (MIC) for VRE isolates from different patient populations.

Main Methods:

  • Determined neomycin MIC for 27 VRE isolates from leukemia and renal unit patients.
  • Assessed VRE recovery on neomycin agar versus blood agar.

Related Experiment Videos

  • Studied eight additional VRE isolates previously recovered from renal unit patients on neomycin agar.

    • Main Results:

    • Eleven of 14 VRE isolates from leukemia patients showed equal recovery on both media with MICs > 64 mg/l.
    • Three VRE isolates from leukemia patients had reduced recovery on neomycin agar and an MIC of 8 mg/l.
    • Eleven of 13 VRE isolates from renal unit patients showed a 4-5 log10 reduction in recovery on neomycin agar; all eight previously selected isolates had MICs > 64 mg/l.

    Conclusions:

    • Neomycin concentrations in selective media may impact VRE recovery.
    • VRE isolates exhibit variable neomycin resistance.
    • Comparative studies of selective screening media are crucial for accurate VRE surveillance.