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Updated: Aug 9, 2026
Murine Model of CD40-activation of B cells
Published on: March 6, 2010
Structure and expression of murine CD30 and its role in cytokine production
M A Bowen1, R K Lee, G Miragliotta
1Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121, USA.
Abstract:
Murine CD30 cDNA predicts a protein of 498 amino acids with homology to the TNF receptor family of proteins characterized by repeated cysteine-rich motifs in the extracellular domain. Murine CD30, although homologous to human CD30, has a 90 amino acid gap in an extracellular region that appears to be duplicated in human CD30. Murine CD30 cDNA was shown to be functional through the production of a soluble murine Ig fusion protein (CD30-Ig) that was active in binding to cells that expressed CD30 ligand. CD30-Ig also served as an immunogen for the production of hamster anti-mouse CD30 mAbs, which recognized both CD30 expressed by murine lymphocytes and CD30 expressed by cells transfected with murine CD30 cDNA. CD30 mRNA is highly expressed in the thymus and in activated spleen cells, but not in other tissues tested. In anti-CD3-activated spleen cells, CD30 ligand is expressed primarily by CD4+ T cells, with peak expression at days 1 and 2, whereas CD30 is expressed primarily by CD8+ T cells, with peak expression on days 4 and 5. Stimulation of CD30 by plate-bound anti-CD30 directly signaled for IL-5 but not IFN-gamma production by CD30+ CTL lines. These studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 signaling may be pivotal in the pattern of cytokine production by T cells.
Insights
Murine CD30, a tumor necrosis factor receptor family member, is expressed on T cells and influences cytokine production. This research details its function and expression patterns in immune responses.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- CD30 is a member of the tumor necrosis factor receptor superfamily.
- Understanding CD30's role in immune regulation is crucial for T cell biology.
Purpose of the Study:
- To characterize murine CD30 (mCD30) and its function.
- To investigate the expression patterns of mCD30 and its ligand in T cells.
- To determine the signaling pathways activated by mCD30 stimulation.
Main Methods:
- CDNA cloning and sequencing of murine CD30.
- Production and functional analysis of soluble CD30-Ig fusion protein.
- Generation of anti-mouse CD30 monoclonal antibodies (mAbs).
- Analysis of CD30 and CD30 ligand mRNA expression in various tissues and activated T cells.
- Cytokine production assays (IL-5, IFN-gamma) upon CD30 stimulation.
Main Results:
- Murine CD30 cDNA encodes a 498-amino acid protein homologous to human CD30, with distinct extracellular domain features.
- Soluble CD30-Ig fusion protein binds CD30 ligand and elicits anti-CD30 mAbs.
- CD30 mRNA is highly expressed in thymus and activated spleen cells.
- CD30 ligand peaks on CD4+ T cells early after activation, while CD30 peaks on CD8+ T cells later.
- CD30 stimulation directly induces IL-5 production in CD30+ cytotoxic T lymphocyte (CTL) lines.
Conclusions:
- Murine CD30 is a functional receptor with homology to human CD30.
- CD30 and its ligand exhibit distinct temporal and cellular expression patterns during T cell activation.
- CD30 signaling directly influences cytokine secretion, specifically IL-5, suggesting a pivotal role in T cell-mediated immune responses.
