Rapid differentiation of Prevotella intermedia and P. nigrescens by 16S rDNA PCR-RFLP

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Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
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Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

S E Milsom1, S V Sprague, D Dymock

  • 1Department of Oral and Dental Science, University of Bristol.

Journal of Medical Microbiology
|January 1, 1996
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...