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Expression of messenger RNA encoding a paraneoplastic cerebellar degeneration-associated antigen in the rat
K Sakai1, Y Kitagawa, T Ogasawara
1Department of Neurology, Kanazawa Medical University, Ishikawa, Japan.
Abstract:
The PCD17 protein is a paraneoplastic cerebellar degeneration (PCD)-associated neural autoantigen which is recognized by autoantibodies in sera of PCD patients. The localization of the PCD17 mRNA was examined by in situ hybridization histochemistry using a PCD17 cRNA probe. In the cerebellum, the localization of the transcript was in agreement with the distribution of PCD17-immunoreactivity. On the other hand, intense hybridization signals were detected in most of cells in the hippocampus in which notable PCD17-immunoreactivity was undetected. These observations suggest that some negative translational or post-transcriptional mechanisms of the PCD17 mRNA may be involved in neurons of the hippocampus.