Related Experiment Videos
Regulated expression and function of CD122 (interleukin-2/interleukin-15R-beta) during lymphoid development
T Reya1, J A Yang-Snyder, E V Rothenberg
1Department of Microbiology, University of Pennsylvania, Philadelphia 19104-6076, USA.
Blood
|January 1, 1996
Summary
CD122 signaling is crucial for lymphocyte development. This receptor is present on hematopoietic stem cells and T-cell progenitors, indicating its role in regulating immune cell expansion and differentiation.
Area of Science:
- Immunology
- Developmental Biology
Background:
- Signaling via CD122, the interleukin-2 (IL-2)/IL-15 receptor beta-chain, is investigated for its role in lymphocyte precursor regulation.
- Understanding CD122's function is key to comprehending immune system development.
Purpose of the Study:
- To determine if CD122 signaling regulates the expansion and differentiation of lymphocyte precursors.
- To characterize CD122 expression and its influence on developing lymphoid cells.
Main Methods:
- Analysis of CD122 expression on hematopoietic stem cells (Sca1+Lin-) and lymphoid progenitors in fetal liver and thymus.
- Localization of CD122 and IL-2 mRNA in embryonic tissues.
- Assessment of B cell development in IL-2-deficient mice.
Main Results:
- CD122 was found on hematopoietic stem cells and a majority of intrathymic T-cell progenitors.
- CD122 expression was detected on early B220+ B cell precursors (prepro B cells) that proliferate in response to IL-2.
- IL-2-deficient mice exhibited a progressive loss of B cells, supporting the IL-2/IL-2R pathway's role.
Conclusions:
- CD122 signaling plays a significant role in normal lymphocyte development in vivo.
- The findings suggest CD122 is integral to both T-cell and B-cell lineage development.