Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasma input function determination for PET using a commercial laboratory robot

D L Alexoff1, C Shea, J S Fowler

  • 1Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973, USA.

Nuclear Medicine and Biology
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stimulant-induced dopamine increases are markedly blunted in active cocaine abusers.

Molecular psychiatry·2014
Same author

Positron emission tomography in the study of aging and senile dementia.

Neurobiology of aging·2013
Same author

Is there evidence of birth control in late Imperial China?

Population and development review·2008
Same author

Therapeutic doses of amphetamine or methylphenidate differentially increase synaptic and extracellular dopamine.

Synapse (New York, N.Y.)·2005
Same author

PET tracers and radiochemistry.

Annals of the Academy of Medicine, Singapore·2004
Same author

Study of brain uptake and biodistribution of [11C]toluene in non-human primates and mice.

Life sciences·2002
Same journal

Enhanced pre-therapy dosimetry for SSTR PET: A head-to-head preclinical assessment of [<sup>89</sup>Zr]Zr-DFO-TOC and [<sup>89</sup>Zr]Zr-DFO-TATE.

Nuclear medicine and biology·2026
Same journal

Pharmacokinetics and dosimetry of radiolabeled FAP-targeting single-domain antibody 4AH29 in Göttingen minipigs.

Nuclear medicine and biology·2026
Same journal

Comparative intra-articular retention of [<sup>177</sup>Lu]Lu-MAA and <sup>90</sup>Y resin in a rat knee model.

Nuclear medicine and biology·2026
Same journal

Evaluating the tumour detection efficacy of [<sup>18</sup>F]Fluorthanatrace positron emission tomography in a metastatic lung cancer animal model.

Nuclear medicine and biology·2026
Same journal

Development of a pyrogallol-formaldehyde resin column for one-step pre-purification of <sup>68</sup>Ge/<sup>68</sup>Ga generator eluates: Enhanced [<sup>68</sup>Ga]Ga-DOTA-TATE radiolabeling yield by selective removal of tetravalent cationic impurities.

Nuclear medicine and biology·2026
Same journal

[<sup>68</sup>Ga]Ga-NOTA-ATH001 positron emission tomography as a general tool for non-invasive detection of platelet derived growth factor receptor beta in mesenchymal cells.

Nuclear medicine and biology·2026
See all related articles

This study automated radiochemical assays for positron emission tomography (PET) studies using a laboratory robot. The system rapidly determines plasma radioactivity and parent radiotracer levels, enhancing efficiency and reducing human contact.

Area of Science:

  • Nuclear Medicine
  • Analytical Chemistry
  • Laboratory Automation

Background:

  • Quantitative Positron Emission Tomography (PET) studies require accurate determination of plasma input function.
  • Traditional radiochemical assays for plasma analysis are time-consuming and involve direct human handling of radioactive materials.
  • Automation is crucial for improving throughput and safety in radiopharmaceutical research.

Purpose of the Study:

  • To develop and validate an automated robotic system for rapid radiochemical assays in quantitative PET studies.
  • To determine total plasma radioactivity and parent radiotracer concentrations in human and baboon plasma.
  • To assess the efficiency, throughput, and safety of the automated system.

Main Methods:

  • Interfacing a commercial laboratory robot (Zymate PyTechnology II) with custom and standard laboratory equipment.

Related Experiment Videos

  • Programming the robot to perform two key assays: total plasma radioactivity and parent radiotracer determination using solid-phase extraction.
  • Utilizing a Zymark XP robot arm for automated sample handling, centrifugation, pipetting, weighing, and radioactivity counting.

    • Main Results:

    • The automated system achieved a throughput of 14.3 samples/h for total plasma radioactivity and 4-6 samples/h for parent radiotracer assays.
    • Specific percentages of parent radiotracers were quantified for various carbon-11 labeled compounds in baboon and human plasma.
    • The robotic system has been reliably used for over 4 years for routine plasma analyses of 7 different C-11 labeled compounds.

    Conclusions:

    • The automated robotic system provides a rapid, efficient, and safe method for radiochemical assays in quantitative PET studies.
    • Automation significantly reduces human contact with radioactive materials, improving laboratory safety.
    • The system's high throughput and reliability make it suitable for routine analysis of various radiotracers.