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Immunological responses to phospholipase-A of Salmonella typhi
1Department of Microbiology, College of Basic Sciences, Himachal Pradesh Agricultural University, Palampur, India.
Acta Microbiologica Et Immunologica Hungarica
|January 1, 1995
Abstract:
A 39 kD phospholipase-A was purified from Salmonella typhi. The enzyme was efficiently concentrated by precipitation with ammonium sulphate and purified by sequential use of column chromatography on DEAE-Sephacel and Sephadex G-100. Humoral and cellular immune responses were determined against both crude and purified phospholipase-A. Crude fraction was found to be more immunogenic than purified fraction.