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Class I-like CD1A-C do not protect target cells from NK-mediated cytolysis
W J Storkus1, M Wei, P Cresswell
1Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania, USA.
Cellular Immunology
|January 10, 1996
Summary
This study investigated how CD1A, CD1B, and CD1C molecules affect natural killer (NK) cell killing of target cells. Results show that these molecules, when expressed on target cells, do not alter susceptibility to NK cell-mediated lysis.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Classical (HLA-A,B,C) and nonclassical (HLA-G) MHC class I antigens on target cells influence natural killer (NK) cell cytolysis.
- Generally, higher expression of these HLA class I molecules correlates with increased resistance to NK cell-mediated lysis.
Purpose of the Study:
- To evaluate the effect of transfected class I-like CD1A, CD1B, and CD1C molecules on the sensitivity of C1R (HLA-A,B null) target cells to NK cell-mediated killing.
Main Methods:
- Transfection of C1R cells with CD1A, CD1B, and CD1C molecules.
- Assessment of cell surface expression levels of transfected CD1 molecules.
- Evaluation of target cell susceptibility to lysis by fresh and lymphokine-activated NK cells.
Main Results:
- CD1A, CD1B, and CD1C molecules were expressed at physiologically relevant levels on the surface of clonal transfectants.
- No significant change was observed in the susceptibility of these transfectant cells to NK-mediated lysis.
Conclusions:
- Expression of CD1A, CD1B, and CD1C molecules on target cells does not affect their sensitivity to NK cell-mediated killing.
- Unlike classical and nonclassical MHC class I molecules, CD1 molecules do not appear to mediate resistance to NK cell lysis.