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In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
In situ localization of nucleolin in the plant nucleolar matrix
A Minguez1, S Moreno Diaz de la Espina
1Laboratorio de Biología Celular y Molecular Vegetal, Centro de Investigaciones Biológicas, CSIC, Velázquez, Madrid, Spain.
Abstract:
The analysis of isolated nucleolar matrices from onion cells by light and electron microscopy, 2-D separation of proteins, and confocal microscopy has confirmed the existence of an organized nucleolar matrix with a complex protein composition to which are attached the insoluble processing complexes. In the present work, we present evidence from immunoblotting, immunofluorescence, immunogold labeling, and preferential cytochemical staining with bismuth salts that an insoluble fraction of the multifunctional protein nucleolin, is a component of the onion nucleolar matrix, and analyse its ultrastructural distribution in the described domains of the matrix.
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