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Porphyromonas gingivalis FDC381 multiplies and persists within human oral epithelial cells in vitro
P N Madianos1, P N Papapanou, U Nannmark
1Department of Oral Microbiology, Faculty of Odontology, Göteborg University, Sweden.
Abstract:
Porphyromonas gingivalis FDC381 replication and persistence within KB epithelial cells in vitro were studied by means of an antibiotic protection assay and electron microscopy. Intracellular counts decreased during the first 24 h; showed a threefold increase during the second day, indicating intracellular multiplication; and after 8 days declined to levels approximating 40% of the initial invasion. The ability of P. gingivalis to persist and multiply within epithelial cells may constitute a pathogenic mechanism in periodontal disease.
Insights
Porphyromonas gingivalis can survive and multiply inside human epithelial cells, potentially contributing to periodontal disease. This bacterial persistence within cells highlights a key virulence factor.
Area of Science:
- Microbiology
- Cell Biology
- Periodontology
Background:
- Porphyromonas gingivalis is a key pathogen implicated in chronic periodontitis.
- Understanding bacterial interactions with host cells is crucial for elucidating disease mechanisms.
Purpose of the Study:
- To investigate the in vitro replication and persistence of Porphyromonas gingivalis FDC381 within KB epithelial cells.
- To determine the intracellular survival dynamics of P. gingivalis.
Main Methods:
- Utilized an antibiotic protection assay to quantify intracellular bacteria.
- Employed electron microscopy to visualize bacterial presence and interaction within host cells.
Main Results:
- Intracellular P. gingivalis counts initially decreased within 24 hours post-infection.
- A threefold increase in intracellular bacterial numbers was observed on day two, indicating multiplication.
- After 8 days, bacterial levels stabilized at approximately 40% of the initial invasion count, demonstrating persistence.
Conclusions:
- Porphyromonas gingivalis exhibits significant intracellular replication and long-term persistence within epithelial cells.
- The ability of P. gingivalis to survive and multiply intracellularly represents a potential pathogenic mechanism in the development of periodontal disease.