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A Bacillus subtilis malate dehydrogenase gene

S Jin1, M De Jesús-Berríos, A L Sonenshein

  • 1Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

Journal of Bacteriology
|January 1, 1996
PubMed
Summary

A Bacillus subtilis gene, citH, is essential for aspartate production during sporulation. Its disruption halts spore formation, highlighting its role in this developmental process.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Bacillus subtilis is a model organism for studying bacterial development.
  • Sporulation is a complex differentiation process involving significant metabolic changes.
  • The genes for citrate synthase and isocitrate dehydrogenase are involved in the TCA cycle.

Purpose of the Study:

  • To investigate the function of the malate dehydrogenase gene (citH) in Bacillus subtilis.
  • To determine the role of citH in aspartate metabolism and sporulation.

Main Methods:

  • Gene disruption of citH in Bacillus subtilis.
  • Phenotypic analysis of citH mutant strains.
  • Assessment of aspartate auxotrophy and sporulation efficiency.

Main Results:

  • The citH gene was located downstream of citrate synthase and isocitrate dehydrogenase genes.
  • Disruption of citH resulted in partial auxotrophy for aspartate.
  • citH mutant cells showed a requirement for aspartate during sporulation and were blocked at a late stage in its absence.

Conclusions:

  • The Bacillus subtilis citH gene encodes malate dehydrogenase and is crucial for aspartate biosynthesis.
  • Aspartate is essential for late-stage spore formation in Bacillus subtilis.
  • The citH gene plays a vital role in the metabolic adaptation required for successful sporulation.

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