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Related Experiment Videos

Salmonella enteritidis agfBAC operon encoding thin, aggregative fimbriae

S K Collinson1, S C Clouthier, J L Doran

  • 1Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.

Journal of Bacteriology
|February 1, 1996
PubMed
Summary

Salmonella enteritidis SEF17 fimbriae and E. coli curli share significant genetic and protein similarities. This suggests a common evolutionary origin for these bacterial adhesion structures.

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Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Molecular Biology

Background:

  • Salmonella enteritidis produces SEF17 fimbriae, crucial for adhesion.
  • These fimbriae are composed of polymerized AgfA fimbrin proteins.

Purpose of the Study:

  • To investigate the genetic organization and expression of SEF17 fimbriae in Salmonella enteritidis.
  • To compare the SEF17 fimbriae system with Escherichia coli curli.

Main Methods:

  • DNA sequence analysis of the agfBAC gene cluster.
  • In vitro expression studies using pUC18 derivatives (pHAG, pDAG).
  • Primer extension and Northern blot analyses for transcription studies.

Main Results:

  • Identified the agfBAC gene cluster encoding AgfA fimbrin and a minor AgfB protein.

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  • AgfA showed high sequence similarity to E. coli CsgA.
  • Transcription analysis revealed a common promoter region for agfA and agfB, analogous to E. coli curli operons.
  • A potential stem-loop structure was identified in the agfA-agfC intercistronic region.

    • Conclusions:

    • Salmonella enteritidis SEF17 fimbriae and E. coli curli exhibit remarkable similarity in fimbrin protein sequence and genetic organization.
    • These similarities indicate a shared evolutionary ancestry between these bacterial adhesion structures.