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Related Experiment Videos

Aromatase expression in endometriosis

L S Noble1, E R Simpson, A Johns

  • 1Cecil H, and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235, USA.

The Journal of Clinical Endocrinology and Metabolism
|January 1, 1996
PubMed
Summary
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Aromatase P450 (P450arom) expression was detected in endometriosis tissues and eutopic endometrium from patients, but not in healthy controls. This suggests altered estrogen production and cytokine regulation in endometriosis.

Area of Science:

  • Reproductive Endocrinology
  • Molecular Biology
  • Gynecologic Oncology

Background:

  • Aromatase P450 (P450arom), encoded by CYP19, converts C19 steroids to estrogens, primarily in the ovary and placenta.
  • P450arom expression is found in uterine tumors like leiomyomas and endometrial cancer, but is undetectable in normal endometrial and myometrial tissues.
  • The role of P450arom in endometriosis, a condition involving estrogen-dependent tissue growth, remains largely uncharacterized.

Purpose of the Study:

  • To investigate the presence or absence of aromatase expression in peritoneal endometriotic implants.
  • To determine aromatase expression in the eutopic endometrium of women diagnosed with endometriosis.
  • To explore potential regulatory mechanisms, including cytokine involvement, for aromatase expression in endometriosis.

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Main Methods:

  • Competitive RT-PCR with an internal standard was used to quantify P450arom transcripts in RNA isolated from endometriotic implants and eutopic endometrial tissues.
  • Tissues from 14 patients with endometriosis and control tissues from 7 disease-free women were analyzed.
  • RT-PCR was also employed to examine the expression of IL-6 and IL-11 messenger RNA in endometriotic and eutopic endometrial samples.

Main Results:

  • P450arom transcripts were detected in all analyzed endometriotic implants and eutopic endometrial tissues from women with endometriosis.
  • P450arom messenger RNA was not detectable in normal endometrial or endometriosis-free peritoneal tissues.
  • IL-6 and IL-11 transcripts were detected in all endometriotic tissues and in the eutopic endometrial sample from a patient with endometriosis.

Conclusions:

  • Eutopic endometrial tissues and endometriotic implants from patients with endometriosis exhibit distinct biochemical profiles compared to normal tissues.
  • The presence of aromatase expression in endometriosis may facilitate estrogen production, potentially promoting implant growth and peritoneal implantation.
  • Increased IL-6 and IL-11 expression suggests these cytokines may play a role in regulating P450arom expression in endometriosis.