Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Angiotensin II type 2 receptor mediates programmed cell death

T Yamada1, M Horiuchi, V J Dzau

  • 1Falk Cardiovascular Research Center, Stanford University School of Medicine, CA 94305-5246, USA.

Proceedings of the National Academy of Sciences of the United States of America
|January 9, 1996
PubMed
Summary

The angiotensin II type 2 (AT2) receptor mediates programmed cell death (apoptosis) by dephosphorylating mitogen-activated protein kinase (MAP kinase). This finding reveals a novel role for the AT2 receptor in cellular regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of Porriolide, a New Metabolite from Alternaria porri.

Bioscience, biotechnology, and biochemistry·2016
Same author

Isolation and Identification of 6-(3',3'-Dimethylallyloxy)-4-methoxy-5-methylphthalide from Alternaria porri.

Bioscience, biotechnology, and biochemistry·2016
Same author

The p38α mitogen-activated protein kinase is a key regulator of myelination and remyelination in the CNS.

Cell death & disease·2015
Same author

Peptidoglycan inhibits progesterone and androstenedione production in bovine ovarian theca cells.

Toxicology in vitro : an international journal published in association with BIBRA·2014
Same author

Relationship between aerobic endurance training and dynamic cerebral blood flow regulation in humans.

Scandinavian journal of medicine & science in sports·2013
Same author

Predominant involvement of the cerebellum in guinea pigs infected with bovine spongiform encephalopathy (BSE).

Journal of comparative pathology·2010

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Physiology

Background:

  • The function of the angiotensin II type 2 (AT2) receptor is largely unknown.
  • AT2 receptor expression is high in fetal tissues and limited in adults, with exceptions like the brain.

Purpose of the Study:

  • To investigate the cellular function of the AT2 receptor.
  • To elucidate the molecular mechanisms underlying AT2 receptor-mediated effects.

Main Methods:

  • Utilized PC12W and R3T3 cell lines expressing AT2 receptors.
  • Investigated the role of mitogen-activated protein kinase (MAP kinase) dephosphorylation.
  • Employed vanadate (protein-tyrosine-phosphatase inhibitor) and antisense oligonucleotide to MAP kinase phosphatase 1.

Related Experiment Videos

Main Results:

  • Demonstrated that AT2 receptor activation induces apoptosis in PC12W and R3T3 cells.
  • Showed that AT2 receptor-mediated apoptosis involves MAP kinase dephosphorylation.
  • Identified MAP kinase phosphatase 1 as a key mediator of AT2 receptor-induced apoptosis.

Conclusions:

  • The AT2 receptor mediates apoptosis through the activation of protein-tyrosine-phosphatases, including MAP kinase phosphatase 1.
  • This apoptotic function of the AT2 receptor may be significant in developmental biology and disease pathophysiology.