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Aggregation-promoting factor in pig intestinal Lactobacillus strains
V Kmet1, M L Callegari, V Bottazzi
1Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Letters in Applied Microbiology
|December 1, 1995
Summary
Intestinal lactobacilli from weaned pigs showed autoaggregation due to a protein factor. This protein in Lactobacillus reuteri strains was identified and showed coaggregation with Escherichia coli.
Area of Science:
- Microbiology
- Animal Science
- Biochemistry
Background:
- Autoaggregation is common in intestinal lactobacilli from weaned pigs.
- The mechanism of aggregation in some strains involves a proteinaceous factor.
Purpose of the Study:
- To investigate the mechanism of autoaggregation in intestinal lactobacilli from weaned pigs.
- To identify the aggregation-promoting factor in specific Lactobacillus reuteri strains.
Main Methods:
- Isolation and characterization of intestinal lactobacilli from weaned pigs.
- Detection of aggregation-promoting protein using cross-reaction with specific antibodies.
- Assessment of coaggregation reactions with Escherichia coli strains.
Main Results:
- Two strains of Lactobacillus reuteri exhibited autoaggregation mediated by a protein factor.
- A 32 kDa aggregation-promoting protein was identified in these strains.
- Lactobacillus reuteri strains showed coaggregation with both pathogenic and non-pathogenic Escherichia coli.
Conclusions:
- A proteinaceous factor mediates autoaggregation in specific Lactobacillus reuteri strains from pigs.
- The identified protein may play a role in the interaction of lactobacilli with other gut bacteria, including Escherichia coli.