Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detection of transcripts and binding sites for colony-stimulating factor-1 during bone development

W Hofstetter1, A Wetterwald, M G Cecchini

  • 1Department of Pathophysiology, University of Bern, Switzerland.

Bone
|August 1, 1995
PubMed
Summary

Colony-stimulating factor-1 (CSF-1) is crucial for osteoclast development during bone formation. This study tracked CSF-1 expression and binding sites in embryonic mouse metatarsals, revealing its role in osteoclast precursor differentiation and bone excavation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Immune Profiling of Murine and Human Pancreatic Cancer Precursors Reveals Species- and Lesion-Dependent Immune Microenvironments.

Laboratory investigation; a journal of technical methods and pathology·2026
Same author

Definition of treatment-resistant late-life depression: Conclusions from a European Task Force Delphi process.

European psychiatry : the journal of the Association of European Psychiatrists·2026
Same author

Contemporary outcomes of left thoraco-abdominal esophagectomy due to cancer in the esophagus or gastroesophageal junction, a multicenter cohort study.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2024
Same author

A soluble LAG-3 protein (eftilagimod alpha) and an anti-PD-L1 antibody (avelumab) tested in a phase I trial: a new combination in immuno-oncology.

ESMO open·2023
Same author

Early Initiation of Sacubitril/Valsartan in Patients With Acute Heart Failure and Renal Dysfunction: An Analysis of the TRANSITION Study.

Journal of cardiac failure·2023
Same author

Comparison of edoxaban and enoxaparin in a rat model of AlCl<sub>3</sub>-induced thrombosis of the superior sagittal sinus.

Naunyn-Schmiedeberg's archives of pharmacology·2023

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Skeletal Biology

Background:

  • Colony-stimulating factor-1 (CSF-1) is a growth factor for mononuclear phagocytes.
  • CSF-1 is essential for osteoclast formation, a key process in bone remodeling.

Purpose of the Study:

  • Investigate CSF-1 transcript expression during embryonic bone development.
  • Detect CSF-1 binding sites in relation to osteoclastogenesis in vivo.

Main Methods:

  • Utilized embryonic mouse metatarsal rudiments as an in vivo model.
  • Employed in situ hybridization to detect CSF-1 mRNA.
  • Assessed CSF-1 binding sites on embryonic bone tissues.

Main Results:

  • CSF-1 transcripts were found in cells on the metatarsal surface by embryonic day 17.

Related Experiment Videos

  • CSF-1 mRNA-containing cells were detected within mineralized cartilage by day 18.
  • CSF-1 binding sites appeared on periosteal cells at day 17 and invaded mineralized cartilage by day 18.

    • Conclusions:

    • CSF-1 expression and binding sites are dynamically regulated during embryonic bone development.
    • The spatiotemporal localization of CSF-1 supports its critical role in osteoclast precursor recruitment and differentiation for bone formation.