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Hematopoietic defects in mice lacking the sialomucin CD34
J Cheng1, S Baumhueter, G Cacalano
1Department of Molecular Oncology, Genentech, Inc, South San Francisco, CA, 94108, USA.
Blood
|January 15, 1996
Summary
CD34 is crucial for hematopoietic progenitor cell formation during embryonic and adult development. While CD34-deficient mice show reduced progenitor numbers, they maintain normal blood recovery after irradiation, suggesting compensatory mechanisms.
Area of Science:
- Hematology
- Developmental Biology
- Immunology
Background:
- Hematopoietic stem cells (HSCs) reconstitute blood lineages but are hard to identify.
- CD34, a stem cell-associated antigen, is expressed on HSCs and vascular endothelial cells.
- The function of CD34 in hematopoiesis and vascular development is largely unknown.
Purpose of the Study:
- To investigate the role of CD34 in hematopoiesis.
- To analyze the impact of CD34 deficiency on embryonic and adult blood development.
Main Methods:
- Generation of CD34-null embryonic stem cells and mice.
- Analysis of hematopoietic development in embryoid bodies and fetal tissues.
- Assessment of colony-forming activity of hematopoietic progenitor cells.
- Evaluation of blood cell recovery after irradiation in CD34-deficient mice.
Main Results:
- CD34-null ES cells showed delayed erythroid and myeloid differentiation.
- Embryonic CD34-deficient mice had reduced hematopoietic progenitor numbers.
- Adult CD34-deficient mice exhibited reduced progenitor activity but normal blood profiles and recovery post-irradiation.
Conclusions:
- CD34 is important for progenitor cell formation in embryonic and adult hematopoiesis.
- Compensatory mechanisms likely exist in adult CD34-deficient mice for normal blood recovery.