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Related Experiment Videos

Coagulation patterns during deheparinization with immobilized polycation

L K von Segesser1, T Mihailevic, M Tönz

  • 1Clinic for Cardiovascular Surgery, University Hospital, Zürich, Switzerland.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|July 1, 1994
PubMed
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Ex vivo deheparinization using immobilized polycation significantly accelerated activated coagulation time normalization in a bovine model. This method offers a promising alternative to protamine for reversing heparin during perfusion with coated devices.

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Hematology

Background:

  • Systemic heparinization reversal with protamine is complicated by its reaction with both circulating and surface-bound heparin.
  • Heparin surface-coated devices necessitate effective ex vivo deheparinization strategies.
  • Immobilized polycation offers a potential method for heparin absorption.

Purpose of the Study:

  • To evaluate coagulation patterns during ex vivo deheparinization using an immobilized polycation.
  • To assess the efficacy of a novel deheparinization device in a relevant animal model.
  • To compare ex vivo deheparinization with spontaneous heparin reversal.

Main Methods:

  • A heparin surface-coated venovenous pump loop with a plasma separator containing immobilized polycation was used in a bovine model (n=6).

Related Experiment Videos

  • Systemic heparinization (300 IU/kg) was administered, followed by either ex vivo deheparinization or spontaneous evolution (control).
  • Coagulation parameters including activated coagulation time, antithrombin III, and fibrinopeptide A were measured.

    • Main Results:

    • Ex vivo deheparinization significantly accelerated activated coagulation time normalization compared to the control group (184 ± 5 sec vs. 757 ± 43 sec, P < 0.05).
    • No device failures or plasma separator clotting occurred during the procedures.
    • Fibrinopeptide A production was higher in the ex vivo deheparinization group (9.5 ± 3.5 ng/ml vs. 2.7 ± 0.7 ng/ml, P < 0.05), potentially due to surgical field activation.

    Conclusions:

    • Ex vivo deheparinization with immobilized polycation effectively normalizes activated coagulation time.
    • The developed device is safe and patent for use during perfusion.
    • This approach presents a viable alternative for managing heparinization in clinical settings involving heparin-coated devices.