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Characterization of the camel skin cell line Dubca
M Klopries1, U Wernery, O R Kaaden
1Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximilians-University Munich, Germany.
The British Veterinary Journal
|September 1, 1995
Summary
Researchers established a new dromedary camel cell line, Dubca cells, for virological studies. These transformed cells support the propagation of several important animal viruses, offering a valuable research tool.
Area of Science:
- Veterinary Virology
- Cell Biology
- Camelid Research
Background:
- Establishing continuous cell lines from exotic animals like dromedary camels is crucial for understanding their unique biology and diseases.
- Viral propagation studies require well-characterized, stable cell lines that can be cultured long-term.
Purpose of the Study:
- To establish and characterize a permanent cell line from dromedary camel skin fibroblasts.
- To assess the susceptibility of the new cell line to infection by various animal viruses.
Main Methods:
- Skin fibroblasts from a dromedary foetus were cultured and transformed with Simian Virus 40 (SV40).
- The resulting cell line, Dubca cells, was characterized morphologically, cytogenetically, and via DNA fingerprinting.
- Viral susceptibility was tested using Camelpox virus (CaPV), bovine herpesvirus-1 (BHV-1), vesicular stomatitis virus (VSV), and border disease virus (BDV).
Main Results:
- A permanent dromedary camel cell line (Dubca cells) was successfully established and maintained for 95 passages.
- Characterization confirmed cell line identity and stability, including detection of SV40 large T antigen.
- Dubca cells demonstrated susceptibility to CaPV, BHV-1, VSV, and BDV, supporting their replication.
Conclusions:
- Dubca cells represent a valuable, permanent cell line for dromedary camel research.
- This cell line serves as a useful host system for studying and propagating various animal viruses relevant to camelid health.