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Crossreactivity between Dermatophagoides siboney and other house dust mite allergens in sensitized asthmatic patients
R Ferrándiz1, R Casas, S Dreborg
1Department of Allergens, National Center for Bioproducts, Havana, Cuba.
Background:
The role of Pyroglyphid mites in IgE-mediated sensitization has been well established. Although mites belonging to the genus Dermatophagoides dominate in the acarofauna of domestic dust, non-pyroglyphid mites might also be of clinical importance. In Cuba, Dermatophagoides siboney is found in dust coexisting with D. pteronyssinus and Blomia tropicalis. Dermatophagoides farinae is not found. Storage mites, such as Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae, might also be present.
Objectives:
The aim of this study was to investigate the allergenic relationships among the mites present in the environment of allergic patients in Cuba.
Methods:
The crossreactivity between an extract of D. siboney and the above mentioned six mites was studied by specific IgE inhibition, using sera of mite-sensitive patients after incubation with serial dilutions of D. siboney.
Results:
The inhibitory effect of D. siboney extract was more pronounced against D. farinae, 91%, than against D. pteronyssinus, 62%. Specific IgE to B. tropicalis, A. siro, L. destructor and T. putrescentiae were inhibited to a much lesser extent, i.e. between 47 and 58%. The correlation between IgE to D. siboney and IgE to D. pteronyssinus and D. farinae was of 0.92 and 0.90, respectively. A weaker correlation was found between D. siboney and L. destructor (0.7), A. siro (0.67), B. tropicalis (0.54) and T. putrescentiae (0.51).
Conclusion:
Thus, D. siboney seems to be most closely related to D. farinae.
Insights
Dermatophagoides siboney mite extract shows strong cross-reactivity with Dermatophagoides farinae, indicating a close allergenic relationship. This finding is significant for understanding mite allergy in Cuba.
Area of Science:
- Immunology
- Allergology
- Entomology
Background:
- Pyroglyphid mites are known triggers of IgE-mediated sensitization.
- While Dermatophagoides species are common, other mites like Blomia tropicalis and storage mites may also be clinically relevant.
- Dermatophagoides siboney is prevalent in Cuba, coexisting with D. pteronyssinus and B. tropicalis, but not D. farinae.
Purpose of the Study:
- To investigate the allergenic relationships among mites found in the environment of allergic patients in Cuba.
- To determine cross-reactivity patterns between Dermatophagoides siboney and other common mites.
Main Methods:
- Specific IgE inhibition assays were performed using sera from mite-sensitive patients.
- Serial dilutions of Dermatophagoides siboney extract were used to inhibit IgE binding to other mite extracts.
- Cross-reactivity was assessed by measuring the reduction in specific IgE levels.
Main Results:
- Dermatophagoides siboney extract demonstrated significant IgE inhibition against Dermatophagoides farinae (91%) and Dermatophagoides pteronyssinus (62%).
- Lower inhibition percentages were observed for Blomia tropicalis, Acarus siro, Lepidoglyphus destructor, and Tyrophagus putrescentiae (47-58%).
- High correlations were found between IgE to D. siboney and D. farinae (0.90) and D. pteronyssinus (0.92).
Conclusions:
- Dermatophagoides siboney exhibits the closest allergenic relationship with Dermatophagoides farinae.
- These findings contribute to understanding mite allergy profiles in Cuba and potential cross-reactivity implications.