Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stereostructure of Rimocidin

P Sowiński1, J Pawlak, E Borowski

  • 1Department of Drug Technology and Biochemistry, Technical University of Gdańsk, Poland.

The Journal of Antibiotics
|November 1, 1995
PubMed
Summary

Nuclear Magnetic Resonance (NMR) studies determined the precise 3D structure of rimocidin. This research established the absolute configuration of its chiral centers and confirmed the all-trans geometry of its tetraene chromophore.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbiological studies of the round goby (<i>Neogobius melanostomus</i>) from the Puck Bay, southern Baltic Sea.

Polish journal of veterinary sciences·2023
Same author

Saduria entomon infected with Hysterothylacium aduncum found in situ in the stomach of cod (Gadus morhua) from the Baltic Sea.

Journal of helminthology·2017
Same author

Global analysis of gene expression in maize leaves treated with low temperature. II. Combined effect of severe cold (8 °C) and circadian rhythm.

Plant molecular biology·2017
Same author

Postantibiotic effect and postantibiotic sub-MIC effect of LTX-109 and mupirocin on Staphylococcus aureus blood isolates.

Letters in applied microbiology·2017
Same author

Personality traits as an endophenotype in genetic studies on suicidality in bipolar disorder.

Acta neuropsychiatrica·2016
Same author

Antiviral and lung protective activity of a novel respiratory syncytial virus fusion inhibitor in a mouse model.

The European respiratory journal·2010

Area of Science:

  • Natural Products Chemistry
  • Organic Chemistry
  • Structural Biology

Background:

  • Rimocidin is a polyene macrolide antibiotic with potential therapeutic applications.
  • Determining the exact three-dimensional structure of natural products is crucial for understanding their mechanism of action and for synthetic efforts.

Purpose of the Study:

  • To elucidate the absolute configuration of rimocidin's chiral centers.
  • To determine the stereochemistry of the tetraene chromophore in rimocidin.

Main Methods:

  • Advanced Nuclear Magnetic Resonance (NMR) techniques were employed, including DQF-COSY, ROESY, HSQC, HMBC, and 1D-TOCSY experiments.
  • These experiments allowed for detailed structural analysis and stereochemical assignments.

Main Results:

  • The absolute configuration of multiple chiral centers in rimocidin was assigned: 2S, 3R, 9S, 11R, 13S, 14R, 15S, 17R, and 27R.
  • The tetraene chromophore within rimocidin was confirmed to possess an all-trans geometry.

Conclusions:

  • The comprehensive NMR analysis provided definitive structural information about rimocidin.
  • This detailed structural assignment is vital for future drug development and chemical biology studies involving rimocidin.

Related Experiment Videos