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Crystal-cell interaction inhibition by polysaccharides
C F Verkoelen1, J C Romijn, L C Cao
1Department of Urology, AEM-Unit, Erasmus University, Rotterdam, The Netherlands.
Purpose:
We studied the effect of polysaccharides on interactions between calcium oxalate monohydrate (COM) crystals and cultured renal cells.
Materials And Methods:
Monolayers of Madin-Darby canine kidney (MDCK) cells were incubated with radiolabeled crystals in the presence of various concentrations of natural glycosaminoglycans (GAGs) and semisynthetic polysaccharides (SSPs).
Results:
While most GAGs were found to have relatively little effect, SSPs (SP54, G871 and G872) were potent inhibitors of crystal-cell association. Pretreatment of crystals, but not of cells, was similarly effective, suggesting polysaccharide-induced modification of crystal surface properties.
Conclusions:
This result further supports the idea that SSPs, and especially G872, are of potential interest for treatment of recurrent stone disease.