Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endogenous reactive oxygen metabolites mediate sublethal endothelial cell dysfunction during reoxygenation

M T Watkins1, H al-Badawi, R Cardenas

  • 1Department of Surgery, Boston University School of Medicine, USA.

Journal of Vascular Surgery
|January 1, 1996
PubMed
Summary

Hypoxia reduces endothelial cell prostacyclin production. Reoxygenation with catalase or superoxide dismutase restored levels, indicating reactive oxygen metabolites mediate this decrease, possibly from mitochondria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct transvaginal ultrasound-guided sclerotherapy for pelvic venous disease.

Journal of vascular surgery. Venous and lymphatic disorders·2026
Same author

Endovascular management of hypervascular retained products of conception: A comprehensive review.

Radiologia·2026
Same author

Allergen immunotherapy for the control of moderate to severe allergic asthma: an evidence-based conjoint analysis to define candidate patient profiles in Spain and Portugal.

Frontiers in allergy·2025
Same author

Correction: Exchange-bias and magnetic anisotropy fields in core-shell ferrite nanoparticles.

Scientific reports·2025
Same author

Successful reversal of intestinal obstruction by PRRT: two case reports and literature review of PRRT in Small Intestinal Neuroendocrine Tumors with mesenteric fibrosis.

Acta gastro-enterologica Belgica·2025
Same author

An unusual case of high gastrointestinal bleeding after Whipple surgery.

Acta gastro-enterologica Belgica·2024

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Endothelial cells (EC) are crucial for maintaining organ blood flow through the secretion of vasoactive eicosanoids.
  • Prostacyclin is a key eicosanoid produced by EC, regulating vascular tone and inhibiting platelet aggregation.

Purpose of the Study:

  • To investigate the impact of reoxygenation on prostacyclin production by endothelial cells following hypoxic conditions.
  • To identify the role of reactive oxygen metabolites in the observed changes in eicosanoid biosynthesis.

Main Methods:

  • Bovine aortic EC cultures were subjected to normoxia, followed by hypoxia, and then reoxygenation.
  • Reoxygenation was performed with various agents including superoxide dismutase (SOD), catalase, allopurinol, and dinitrophenol to assess their effects.

Related Experiment Videos

Main Results:

  • Hypoxia significantly decreased prostacyclin production to 40% of basal levels.
  • Reoxygenation alone did not restore prostacyclin production.
  • Treatment with SOD or catalase during reoxygenation led to recovery of prostacyclin production to 154% and 105% of basal levels, respectively.
  • Dinitrophenol significantly sustained prostacyclin production recovery throughout reperfusion.

Conclusions:

  • The hypoxia-induced reduction in EC prostacyclin production is not spontaneously recovered during reoxygenation.
  • Reactive oxygen metabolites are implicated as mediators of decreased eicosanoid biosynthesis.
  • Evidence suggests a mitochondrial origin for the oxygen metabolites responsible for suppressing prostacyclin biosynthesis.