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[Studies of thrombocyte function in CPD blood]
Insights
Storing platelet-rich plasma with CPD stabilizer at room temperature for 3 days significantly impairs thrombocyte function. Refrigeration at 4 degrees C preserves some function, with pH influencing the loss.
Area of Science:
- Biochemistry
- Hematology
- Blood Storage
Context:
- Platelet-rich plasma is crucial for hemostasis.
- Effective blood storage requires preserving platelet function.
- CPD stabilizer with adenine sulfate and guanosine is used for erythrocyte preservation.
Purpose:
- To investigate the preservation of global thrombocyte function in platelet-rich CPD plasma during storage.
- To evaluate the impact of storage temperature (4°C vs. room temperature) on thrombocyte function over 3 days.
Summary:
- Platelet-rich CPD plasma was stored for 3 days at 4°C or room temperature.
- Thrombocyte function, assessed by the DKTA method (aggregation-adhesion), was significantly reduced at room temperature.
- Storage at 4°C offered partial preservation of thrombocyte function, with pH being a critical factor.
Impact:
- Demonstrates the detrimental effect of room temperature storage on platelet function in CPD plasma.
- Highlights the importance of temperature control in blood product storage for maintaining hemostatic capacity.
- Suggests that pH significantly influences thrombocyte function loss during storage.
Abstract:
The CPD stabilizer according to Gibson with an addition of 1.25 mMol adeninesulfate and 2.50 mMol guanosine is used in blood storage for better preserving 2.3-bis-phosphoglycerate of erythrocytes. Here platelet-rich CPD plasma was investigated before and during a 3 days storage at 4 degrees C or room temperature with regard to preserving the global thrombocyte function. The latter consists in the ability to seal blood vessels and is tested by means of pressure registration in combined thrombocyte-aggregation-adhesion (DKTA method) as an ability to close the pores of a sieve by adding 10(-5) mM/l of ADP. At room temperature this thrombocyte function is approximately 0 following 3 days of storage in CPD plasma excess without shaking. When stored at 4 degrees C it is preserved to a slight degree. Loss of thrombocyte function will depend on pH, thus being particularly evident at room temperature.