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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Macrolide resistance in Staphylococcus aureus: inducers of macrolide resistance

N E Allen

    Antimicrobial Agents and Chemotherapy
    |April 1, 1977
    PubMed

    Insights

    Certain macrolide antibiotics and celesticetin induce resistance by targeting specific sites. Modifications to erythromycin A (EM) can abolish induction while retaining inhibition, showing distinct mechanisms for these antibiotic effects.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Macrolide-, lincosamide-, and streptogramin B-type (MLS) antibiotics are crucial in treating bacterial infections.
    • Understanding the mechanisms of antibiotic resistance, particularly induction of resistance, is vital for developing new therapeutic strategies.

    Purpose of the Study:

    • To investigate the specific structural requirements of MLS antibiotics for inducing resistance.
    • To determine if antibiotic-induced resistance and inhibition share common molecular targets.

    Main Methods:

    • Testing various MLS antibiotics for their ability to induce [(14)C]leucine incorporation resistant to erythromycin A (EM).
    • Analyzing the structure-activity relationship of EM modifications on inducer and inhibitor properties.

    Main Results:

    • Only 14-membered ring macrolides with a 6-deoxy sugar at C-3 and the lincosamide celesticetin demonstrated inducer activity.
    • Modifications at the C-4'' position of EM's cladinose abolished inducer activity but not inhibitory effects.

    Conclusions:

    • Inducer and inhibitor activities of MLS antibiotics can be dissociated, suggesting different molecular targets.
    • Distinct binding sites are likely involved in ribosome inhibition and resistance induction by these antibiotics.

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