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Acquisition of CD40 expression during murine B-cell differentiation
A Grandien1, A Brás, C Martinez
1Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
Scandinavian Journal of Immunology
|January 1, 1996
Summary
CD40 expression in mice is limited to B cells after gene rearrangement. Levels increase during B cell maturation, with highest expression found in lymph nodes and Peyer's patches.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD40 is a crucial co-stimulatory molecule involved in immune responses.
- Understanding CD40 expression patterns is vital for B cell development and function.
Purpose of the Study:
- To investigate the expression profile of CD40 on mouse cells.
- To determine the stages of B cell maturation where CD40 is expressed.
Main Methods:
- Utilized monoclonal antibodies against CD40 and a soluble CD40-ligand fusion protein for binding assays.
- Analyzed CD40 expression on various mouse cell lines and primary cells from normal mice.
Main Results:
- CD40 expression was not detected on pro- or pre-B cells.
- Expression was restricted to B cells with successful immunoglobulin gene rearrangement.
- CD40 levels increased with B cell maturation, from low levels on pre-B-II cells to high levels on mature B cells.
- Highest CD40 expression was observed in B cells from lymph nodes and Peyer's patches.
Conclusions:
- CD40 expression is a late event in B cell development, coinciding with surface immunoglobulin expression.
- B cell maturation stage and anatomical location significantly influence CD40 expression levels.