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Related Experiment Videos

Bacteriophage T4 as a surface display vector

V P Efimov1, I V Nepluev, V V Mesyanzhinov

  • 1Ivanovsky Institute of Virology, Moscow, Russia.

Virus Genes
|January 1, 1995
PubMed
Summary

Researchers engineered bacteriophage T4 particles to display foreign proteins on their surface. This novel phage display system enhances current capabilities by modifying the fibritin protein.

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Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Bacteriophage T4 is a well-characterized virus.
  • Surface display systems are valuable tools in biotechnology.
  • Fibritin is a minor structural protein of bacteriophage T4.

Purpose of the Study:

  • To develop a novel phage display system using bacteriophage T4.
  • To engineer T4 particles for displaying foreign polypeptides on their surface.

Main Methods:

  • Modification of the T4 fibritin gene (wac) to allow for C-terminal extension.
  • Homologous recombination to introduce the modified gene into the T4 genome.
  • Verification of modified fibritin expression and binding to phage particles.

Main Results:

  • Successfully constructed chimeric bacteriophage T4 particles displaying foreign polypeptides.
  • Demonstrated that fibritin can be lengthened at the C terminus without affecting folding or phage binding.
  • Generated T4 display particles carrying a 53-residue polypeptide, including a 45-residue fragment from hepatitis B virus pre-S2 region.

Conclusions:

  • The described method provides a new approach for bacteriophage T4-based display systems.
  • This technique expands the utility of T4 phage as a display vector.
  • The engineered T4 particles show potential for various biotechnological applications.

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