Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[In vitro endothelial dysfunction after cold storage: comparison with various preservative solutions]

F Dagenais1, J Buluran, R Cartier

  • 1Département de Chirurgie, Institut de Cardiologie de Montréal, Québec, Canada.

Annales De Chirurgie
|January 1, 1995
PubMed
Summary

Organ preservation solutions impact endothelial and smooth muscle function. University of Wisconsin (UW) or Krebs Ringer (KR) solutions better preserve endothelial function during cold storage compared to normal saline (NS) or rat blood (RB).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A kilonova as the electromagnetic counterpart to a gravitational-wave source.

Nature·2017
Same author

THE EFFECT OF FACTOR SCORES, GUTTMAN SCORES, AND SIMPLE SUM SCORES ON THE SIZE OF F RATIOS IN AN ANALYSIS O F VARIANCE DESIGN.

Multivariate behavioral research·2016
Same author

Influence of atrial fibrillation in trans-catheter aortic valve replacement.

Minerva medica·2014
Same author

A three-layer model for buckling of a human aortic segment under specific flow-pressure conditions.

International journal for numerical methods in biomedical engineering·2014
Same author

The effect of aortic wall and aortic leaflet stiffening on coronary hemodynamic: a fluid-structure interaction study.

Medical & biological engineering & computing·2013
Same author

Therapeutic vascular compliance change may cause significant variation in coronary perfusion: a numerical study.

Computational and mathematical methods in medicine·2012

Area of Science:

  • Vascular Biology
  • Organ Transplantation
  • Preservation Medicine

Background:

  • Optimal preservation solutions for maintaining endothelial function during organ transplant cold storage remain undefined.
  • Cold storage is critical for organ viability but can induce vascular dysfunction.

Purpose of the Study:

  • To evaluate the efficacy of different preservation solutions in preserving endothelial and smooth muscle function of rat aortic rings during cold storage.
  • To identify the best solution for minimizing cold storage-induced vascular damage.

Main Methods:

  • Rat aortic rings (n=28) were cold-stored for 4 hours in Krebs Ringer (KR), normal saline (NS), rat blood (RB), or University of Wisconsin (UW) solution.
  • Endothelial-dependent relaxation (acetylcholine, adenosine diphosphate, histamine) and smooth muscle function (norepinephrine, sodium nitroprusside) were assessed.

Related Experiment Videos

  • A control group (CTL, n=7) without storage was used for comparison.

    • Main Results:

    • All storage solutions significantly decreased endothelial-dependent relaxations compared to controls.
    • Normal saline (NS) and rat blood (RB) storage resulted in significantly worse endothelial-dependent relaxation to acetylcholine compared to UW solution.
    • All stored groups exhibited hypercontractility to norepinephrine, indicating impaired smooth muscle function.
    • KR and UW solutions demonstrated less endothelial dysfunction compared to NS and RB.

    Conclusions:

    • Cold storage negatively impacts both endothelial and smooth muscle function in vascular tissue.
    • The University of Wisconsin (UW) solution and Krebs Ringer (KR) offer superior endothelial function preservation compared to normal saline (NS) or rat blood (RB) during cold storage.
    • Further research is needed to optimize organ preservation strategies to mitigate cold ischemic injury.