Egg dipping in hydrogen peroxide solution to eliminate Salmonella typhimurium from eggshell membranes
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Updated: Nov 6, 2026

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Published on: January 27, 2021
Abstract:
The effectiveness of egg dipping in a 6% hydrogen peroxide solution to eliminate Salmonella typhimurium from eggshell membranes was evaluated. The first step was to assess the water uptake from broiler hatching eggs dipped in tap water once or twice using a positive-pressure differential method in order to determine the best method to use in the disinfection trials. Double dipping increased water uptake by 86% more than single dipping. Dipping S. typhimurium-contaminated eggs twice in a 6% hydrogen peroxide solution reduced the average number of organisms in eggshell membranes by 95% and the number of S. typhimurium-positive eggs by 55% compared with the infected untreated group. Dipping the eggs in a 6% hydrogen peroxide solution did not adversely affect hatchability.
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