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Updated: Aug 5, 2026
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016
Molecular detection and serotypic analysis of enterovirus RNA in archival specimens from patients with acute
F Nicholson1, J F Ajetunmobi, M Li
1Department of Virology, United Medical School, Guy's and St Thomas', (St Thomas' campus) London.
Objective:
To determine whether enterovirus RNA can be demonstrated in archival necropsy material in acute myocarditis.
Design:
Analysis of paraffin embedded myocardial tissue from cases of acute myocarditis.
Setting:
University virology department.
Methods:
Extraction of RNA from tissue followed by polymerase chain reaction (PCR) and DNA sequence analysis.
Patients:
Six patients with histologically proven myocarditis and eight controls.
Results:
Enterovirus RNA was identified in 5 of 6 patients with myocarditis and in none of the controls. The nucleotide sequences of the PCR products showed greatest similarity to group B coxsackieviruses, particularly coxsackievirus B3.
Conclusion:
This study indicates that archival tissue samples, even histologically stained tissue sections, can be used to study the role of enteroviruses in myocardial disease using molecular detection techniques. If a predominant role for coxsackievirus B3 in myocarditis is confirmed by further study, this may have implications for the development of a specific vaccine.
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