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Related Experiment Videos

Complex asparagine-linked oligosaccharides in Mgat1-null embryos

R M Campbell1, M Metzler, M Granovsky

  • 1Howard Hughes Medical Institute, University of California San Diego, La Jolla 92093, USA.

Glycobiology
|July 1, 1995
PubMed
Summary

Complex N-glycans are crucial for mouse embryonic development after pre-implantation stages. Mgat1-null embryos survive early development using maternal N-glycans but fail later when embryonic N-glycans are required.

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Area of Science:

  • Developmental Biology
  • Glycobiology
  • Molecular Genetics

Background:

  • Complex N-glycans are essential for various biological processes.
  • The Mgat1 gene encodes GlcNAc-TI, a key enzyme in complex N-glycan synthesis.
  • Previous studies showed Mgat1-null embryos exhibit abnormalities and lethality post-embryonic day 10.5.

Purpose of the Study:

  • To investigate the temporal and spatial expression of the Mgat1 gene during mouse embryogenesis.
  • To determine the presence and timing of complex N-glycans in Mgat1-null embryos.
  • To understand the role of embryonically derived complex N-glycans in early development.

Main Methods:

  • In situ RNA hybridization to analyze Mgat1 gene expression patterns.
  • Lectin histochemistry to detect complex N-glycans in wild-type and Mgat1-null embryos.

Related Experiment Videos

  • Phenotypic analysis of Mgat1-null embryos at various developmental stages (E3.5 to E9.5).

    • Main Results:

    • Mgat1 transcripts significantly increase between embryonic days 7.0 and 9.5 in normal embryos.
    • Mgat1-null embryos show normal complex N-glycans at E3.5, likely due to maternal contribution.
    • Complex N-glycans become undetectable in Mgat1-null embryos by E7.5, except in visceral endoderm vesicles, and are absent by E9.5.

    Conclusions:

    • Mouse embryonic development up to E7.5 can proceed without embryonically synthesized complex N-glycans, relying on maternal sources.
    • A critical requirement for embryonically derived complex N-glycans emerges post-E7.5, leading to developmental failure in Mgat1-null embryos.
    • This study highlights the essential role of Mgat1-mediated N-glycan synthesis for post-implantation mouse embryonic survival.