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Culturing satellite cells from living single muscle fiber explants
J D Rosenblatt1, A I Lunt, D J Parry
1Muscle Cell Biology Group, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
In Vitro Cellular & Developmental Biology. Animal
|November 1, 1995
Abstract:
Conventional methods for isolating myogenic (satellite) cells are inadequate when only small quantities of muscle, the tissue in which satellite cells reside, are available. We have developed a tissue culture system that reliably permits isolation of intact, living, single muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and differentiation of satellite cells from each fiber; and removal of the fiber from culture for analysis.