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Protective effects of doxycycline in mesenteric ischemia and reperfusion
1Department of Biological Structure and Function, School of Dentistry, Oregon Health Sciences University, Portland 97201, USA.
Research Communications in Molecular Pathology and Pharmacology
|June 1, 1995
Summary
Doxycycline pretreatment improved survival in a rat model of splanchnic artery occlusion shock. This protective effect in circulatory shock was not due to direct sparing of the liver, pancreas, or heart.
Area of Science:
- Physiology
- Pharmacology
- Pathology
Background:
- Splanchnic artery occlusion (SAO) in rats induces severe circulatory shock and multiorgan failure.
- Neutrophils play a critical role in the pathophysiology of SAO-induced shock.
Purpose of the Study:
- To investigate the protective effects of doxycycline (Dc) pretreatment in a rat model of SAO-induced circulatory shock.
- To determine if doxycycline's protective effects are related to specific organ sparing (liver, pancreas, heart).
Main Methods:
- Rats underwent 1-hour splanchnic artery occlusion followed by reperfusion.
- Doxycycline (10mg/kg) was administered 2 hours prior to occlusion.
- Survival time, leukocyte retention, intestinal hemoglobin levels, and serum markers for liver, pancreas, and heart damage were assessed.
Main Results:
- Doxycycline pretreatment significantly increased survival time and reduced leukocyte retention in circulation.
- Doxycycline decreased hemoglobin levels in the small intestine contents.
- Doxycycline did not alter serum markers for liver or pancreas damage but increased a marker for cardiotoxicity (creatinine phosphate kinase).
Conclusions:
- Doxycycline pretreatment confers a protective effect in the SAO rat model of circulatory shock.
- The observed protection is not attributable to direct sparing of the liver, pancreas, or heart.