Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exploring the allowed sequence space of a membrane protein

J Wen1, X Chen, J U Bowie

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles 90095-1570, USA.

Nature Structural Biology
|February 1, 1996
PubMed
Summary

Diacylglycerol kinase from Escherichia coli shows remarkable tolerance to sequence changes, with most residues accepting non-conservative substitutions. Critical active-site residues are located in the second cytoplasmic domain, despite the protein

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shaped by Mountains, Driven by Climate: The Rise of the Neotropical Carex sect. Fecundae.

Annals of botany·2026
Same author

Estimate renal cell carcinoma recurrence rates using electronic health records.

ESMO real world data and digital oncology·2026
Same author

[Biocompatibility and anti-encrustation effect of IDP<sub>FUS</sub> coating-modified ureteral stent materials in SD rat bladder stent retention model].

Zhonghua yi xue za zhi·2026
Same author

Adipose-Derived Stem Cells Pretreated With Phototherapy Promote HUVECs Migration and Angiogenesis by Mediating EYA1 Activation.

Physiological research·2025
Same author

Evaluation of Recyclable Multilayer Packaging Designs Utilising Controlled Interlayer Adhesion.

Experimental mechanics·2025
Same author

Epicardial adipose tissue associated with right cardiac chamber remodelling in patients with connective tissue disease evaluated by cardiac magnetic resonance: a cross-sectional study.

Clinical radiology·2025

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Membrane proteins play crucial roles in cellular functions.
  • Understanding sequence-structure-function relationships in membrane proteins is vital.
  • Diacylglycerol kinase (DGK) is an essential enzyme involved in lipid signaling.

Purpose of the Study:

  • To comprehensively assess the tolerance of diacylglycerol kinase to sequence substitution.
  • To identify conserved and non-conserved residues within the protein.
  • To investigate the distribution of critical residues in different protein domains.

Main Methods:

  • Sequence analysis of diacylglycerol kinase from Escherichia coli.
  • Identification of residues tolerating non-conservative changes.

Related Experiment Videos

  • Mapping of conserved residues in soluble and transmembrane regions.
  • Localization of critical active-site residues.

    • Main Results:

    • Diacylglycerol kinase exhibits extreme tolerance to sequence substitution, with three-quarters of residues tolerating non-conservative changes.
    • Conserved residues are found with similar frequency in both soluble and transmembrane domains.
    • The most critical active-site residues are located in the second cytoplasmic domain.

    Conclusions:

    • The unique structure of the transmembrane portion of diacylglycerol kinase can be encoded by a highly substitution-tolerant sequence.
    • This high tolerance suggests potential for protein engineering and functional studies.
    • Active site localization provides insights into enzyme mechanism and regulation.