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Endothelins inhibit cyclic AMP production in rabbit and human ciliary processes
1Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut, USA.
Abstract:
The effects of endothelins on cyclic AMP production have been studied in rabbit and human ciliary processes. Endothelins inhibit basal, isoproterenol-, and VIP-stimulated cyclic AMP production in rabbit ciliary processes and forskolin-stimulated cyclic AMP production in rabbit and human ciliary processes. EC50s for inhibition of forskolin-stimulated cyclic AMP production using rabbit tissue were similar: ET-1, 70 nM; ET-2, 40 nM; ET-3, 70 nM; maximum inhibition was about 76% for each ligand. These data are consistent with the presence of an ETB receptor. Endothelin-like immunoreactivity in rabbit aqueous humor was measured to be 226 pg/ml.