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Autocrine angiogenic vascular prosthesis with bone marrow transplantation

Y Noishiki¹, Y Tomizawa, Y Yamane

¹First Department of Surgery, Yokohama City University School of Medicine, Japan.

Nature Medicine

|January 1, 1996

Summary

Transplanting bone marrow cells into synthetic vascular grafts promotes capillary growth and complete endothelialization, preventing occlusion. This method enhances graft patency by inducing angiogenesis through the graft walls.

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Area of Science:

Biomaterials Science
Regenerative Medicine
Vascular Surgery

Background:

Synthetic vascular prostheses are prone to blood coagulation and occlusion, especially small-diameter grafts.
Endothelialization is crucial for antithrombogenic properties, requiring angiogenesis within the graft.

Purpose of the Study:

To investigate the efficacy of transplanting bone marrow cells into expanded polytetrafluoroethylene (ePTFE) vascular grafts to promote endothelialization and prevent occlusion.
To evaluate the potential of bone marrow cells to induce capillary growth and maintain graft patency.

Main Methods:

Bone marrow cells were infiltrated into the walls of long-fibril ePTFE vascular grafts.
Grafts were implanted autologously into the abdominal aortic position of 24 dogs.
Control grafts without bone marrow cells were implanted in seven dogs.
Graft patency, endothelialization, and cell survival were assessed up to six months.

Main Results:

Bone marrow cells survived and exhibited exogenous hemopoiesis for up to six months, showing reactivity to basic fibroblast growth factor (bFGF).
All ePTFE grafts treated with bone marrow cells achieved complete endothelialization within three weeks and maintained patency.
Control grafts showed limited endothelialization and a high rate of thrombosis and occlusion.

Conclusions:

Bone marrow transplantation into ePTFE vascular grafts effectively induces angiogenesis and complete endothelialization.
This approach significantly improves graft patency and prevents thrombotic occlusion, offering a promising strategy for small-diameter vascular prostheses.