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Related Experiment Videos

Evaluating the preservative effectiveness of RGP lens care solutions

J Keeven1, S Wrobel, M Portoles

  • 1Microbial Applications Laboratory, Catholic University of America, Washington, DC 20064, USA.

The CLAO Journal : Official Publication of the Contact Lens Association of Ophthalmologists, Inc
|October 1, 1995
PubMed
Summary

Polyhexamethylene biguanide (PHMB) solutions demonstrated superior antimicrobial efficacy against RGP contact lens contaminants. Boston Advance and its enhanced formula effectively killed vegetative cells and fungal spores, outperforming other tested preservatives.

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Area of Science:

  • Ophthalmology
  • Microbiology
  • Materials Science

Background:

  • Rigid gas permeable (RGP) contact lenses require effective antimicrobial preservation in care solutions.
  • Preservative resistance in microorganisms poses a challenge to contact lens hygiene.
  • Evaluating the efficacy of various preservatives is crucial for ensuring eye health.

Purpose of the Study:

  • To assess the antimicrobial preservative efficacy of nine RGP contact lens care solutions.
  • To compare the effectiveness of different preservatives against standard and resistant microbial strains.
  • To identify solutions with optimal broad-spectrum antimicrobial activity.

Main Methods:

  • Laboratory study involving challenge testing of nine RGP contact lens care solutions.

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  • Use of standard test organisms and preservative-resistant microbial strains.
  • Quantification of microbial reduction over a 28-day period.

    • Main Results:

    • Solutions with polyhexamethylene biguanide (PHMB) showed rapid killing of vegetative cells (bacteria and yeast) within 6 hours.
    • Boston Advance Enhanced Comfort Formula demonstrated significant reduction of Aspergillus niger spores.
    • Thimerosal-preserved Soaclens showed limited efficacy against Staphylococcus aureus initially but killed all organisms by day 1.
    • Solutions containing chlorhexidine gluconate (CHG), benzalkonium chloride (BAK), polyquaternium-1 (polyquad), and benzyl alcohol exhibited variable or limited efficacy against adapted strains and fungal spores.

    Conclusions:

    • PHMB-containing solutions are highly effective against vegetative bacteria and yeast in RGP lens care.
    • Boston Advance Enhanced Comfort Formula offers improved efficacy against fungal spores compared to Boston Advance.
    • Some preservatives like BAK and benzyl alcohol show limited effectiveness against adapted microorganisms.
    • Careful selection of RGP contact lens solutions is necessary to ensure adequate microbial control.