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Related Experiment Videos

Differential requirements of CD45 for lymphocyte development and function

Y Y Kong1, K Kishihara, H Sumichika

  • 1Department of Immunology, Kyushu University, Fukuoka, Japan.

European Journal of Immunology
|December 1, 1995
PubMed
Summary

CD45 protein is not essential for B cell function in mice, but it is crucial for T helper cell responses. This highlights differing roles of CD45 in immune cell development and activation.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cellular Biology

Background:

  • CD45 protein tyrosine phosphatase is critical for lymphocyte development and function.
  • CD45 exon 6-deficient mice exhibit a severe block in thymocyte development.
  • B cell development is unaffected in CD45 exon 6-deficient mice.

Purpose of the Study:

  • To investigate the functional role of CD45 in mature B lymphocytes.
  • To determine if CD45 expression is essential for B cell responses in vivo.
  • To compare the requirement of CD45 in T cell versus B cell function.

Main Methods:

  • Analysis of B lymphocyte function in CD45 exon 6-deficient mice.
  • Adoptive transfer of normal CD4+ T lymphocytes into mutant mice.
  • Assessment of T-dependent and T-independent antibody production and class switching.

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Main Results:

  • CD45-defective B lymphocytes from CD45 exon 6-deficient mice exhibit intact functions.
  • Normal T-dependent and T-independent antigen-specific antibody production and class switching were observed in B cells.
  • CD4+ T helper cell function was severely diminished in CD45 exon 6-deficient mice.

Conclusions:

  • CD45 expression on B lymphocytes is not essential for antigen-specific B cell responses in vivo.
  • CD45 plays a critical, non-redundant role in CD4+ T helper cell function.
  • The requirement for CD45 in immune responses differs significantly between T and B lymphocytes.