Related Experiment Videos
Manual versus computer-automated semen analyses. Part III. Comparison of old versus new design MicroCell Chambers
J E Johnson1, W R Boone, D W Blackhurst
1Greenville Hospital System, Reproductive Endocrinology Associates, South Carolina 29605, USA.
Objective:
To determine accuracy and precision of old and new design MicroCell counting Chambers analyzed manually and with a computer-automated semen analyzer (CASA; Hamilton-Thorn Research, Beverly, MA).
Design:
Prospective study using comparative measurements of the concentration of latex beads with old and new design MicroCell Counting Chambers (Conception Technologies, Inc. La Jolla, CA).
Main Outcome Measure:
Beads were counted manually and with CASA to evaluate the accuracy of old and new design 20 MicroCell Chambers.
Results:
The old design 20 MicroCell Chamber demonstrated a significant difference in bead concentration beginning at the 9.9 mm CASA stage position compared with stage positions of 0.0 to 9.5 mm. The new design 20 MicroCell Chamber demonstrated the same difference at the 8.0 mm CASA stage position compared with stage positions 0.0 to 7.6 mm. Similar findings were observed when the beads were analyzed manually.
Conclusions:
When analyzed within a specific range of stage positions, the old and new design 20 MicroCell Chambers are accurate and precise whether analyzed manually or with CASA.