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Rapid identification of Acremonium lolii and Acremonium coenophialum endophytes through arbitrarily primed PCR
D Liu1, R van Heeswijck, G Latch
1Victorian Institute of Animal Science, Attwood, Australia.
FEMS Microbiology Letters
|November 1, 1995
Abstract:
Using a random decamer 5'-CCGAGGTGAC-3' in an arbitrarily primed PCR, similar band patterns were observed between Acremonium lolii and A. coenophialum DNA, which were somewhat different from those formed by other fungal DNA. Despite sharing bands of around 0.7, 0.9 and 2.1 kb, A. lolii can be distinguished from A. coenophialum by the presence of an additional band at around 0.5 kb in the arbitrarily primed PCR.