Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Antibiotic-resistance cassettes for Bacillus subtilis

A M Guérout-Fleury¹, K Shazand, N Frandsen

¹Institut de Biologie Physico-Chimique, Paris, France.

|December 29, 1995

Summary

Antibiotic resistance genes from Escherichia coli were cloned into Bacillus subtilis. This allows for the creation of tagged chromosomal disruptions for genetic research in Bacillus subtilis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Usefulness of the European Epidemic Intelligence Information System in the management of an outbreak of listeriosis, Belgium, 2011.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2012

Same author

[Follow-up of the first case of Mycobacterium ulcerans infection documented by PCR, genotyping and culture in the Republic of Congo-Brazzaville].

Medecine tropicale : revue du Corps de sante colonial·2008

Same author

Identification of a new variable number tandem repeat locus in Mycobacterium ulcerans for potential strain discrimination among African isolates.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2007

Same author

Porphyrin-related photosensitizers for cancer imaging and therapeutic applications.

Journal of microscopy·2005

Same author

FOXO1 and c-jun transcription factors mRNA are modulated in endometriosis.

Molecular human reproduction·2004

Same author

Large scale validation of human N-myc downstream-regulated gene (NDRG)-1 expression in endometrium during the menstrual cycle.

Molecular human reproduction·2003

Area of Science:

Molecular Biology
Microbial Genetics
Bacterial Genetics

Background:

Antibiotic resistance genes are crucial for bacterial survival and are often located on plasmids.
Escherichia coli plasmids are widely used for cloning and genetic manipulation.
Bacillus subtilis is a model organism for Gram-positive bacterial genetics.

Purpose of the Study:

To develop a method for creating tagged chromosomal disruptions in Bacillus subtilis.
To utilize antibiotic resistance gene cassettes for genetic engineering in Bacillus subtilis.
To facilitate the study of gene function in Bacillus subtilis through targeted gene disruption.

Main Methods:

Cloning of antibiotic resistance genes (erythromycin, kanamycin, tetracycline, spectinomycin) into Escherichia coli plasmid vectors.
Insertion of these resistance gene cassettes into cloned Bacillus subtilis genes.
Recombination into Bacillus subtilis and selection using specific antibiotics to create chromosomal disruptions.

Main Results:

Successfully cloned antibiotic resistance gene cassettes into E. coli vectors.
Demonstrated the ability to insert these cassettes into B. subtilis genes.
Established a method for creating antibiotic-selectable tagged chromosomal disruptions in B. subtilis.

Conclusions:

The developed method provides a versatile tool for creating targeted gene knockouts in Bacillus subtilis.
This technique enables the study of essential genes and complex genetic pathways in B. subtilis.
The use of antibiotic resistance markers simplifies the selection process for genetic modifications in B. subtilis.

Related Experiment Videos