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Structure and gene expression of avian cyclin D2
1Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, TN 38101, USA.
Gene
|December 29, 1995
Summary
Researchers identified avian cyclin D2 (Cyl D2) in chickens, finding it highly conserved with mouse and human versions, particularly in the Cyl-box domain. This suggests a crucial role for Cyl D2 in avian immune cells.
Area of Science:
- Molecular Biology
- Immunology
- Genetics
Background:
- Cyclins are key regulators of the cell cycle.
- Cyclin D proteins (D1, D2, D3) are important in cell cycle progression and differentiation.
- Understanding avian cyclin D2 provides insights into immune cell development.
Purpose of the Study:
- To isolate and characterize avian cyclin D2 (Cyl D2)-encoding cDNA.
- To investigate the sequence conservation of avian Cyl D2 with mammalian counterparts.
- To determine the expression pattern of CYL2 mRNA in avian immune cells.
Main Methods:
- Isolation of cDNA clones from a chicken T-cell library.
- Sequence analysis and homology comparison with known cyclin D genes.
- Northern blot analysis to detect CYL2 mRNA expression.
Main Results:
- Avian cyclin D2 (Cyl D2)-encoding cDNA clones were successfully isolated.
- Sequence analysis revealed high conservation with mouse and human Cyl D2, especially in the Cyl-box domain.
- A single 6.0-kb CYL2 mRNA transcript was detected in both avian B- and T-cells.
Conclusions:
- Avian Cyl D2 shares significant homology with mammalian cyclin D2, indicating conserved function.
- The Cyl-box domain is highly conserved across species, highlighting its functional importance.
- CYL2 mRNA is expressed in avian immune cells, suggesting a role in their regulation.