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CD45RO expression on bovine T cells: relation to biological function

G P Bembridge1, N D MacHugh, D McKeever

  • 1Institute for Animal Health, Compton Laboratory, UK.

Immunology
|December 1, 1995
PubMed
Summary
This summary is machine-generated.

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A novel antibody identified the CD45RO isoform in cattle, revealing distinct T-cell functions. CD45RO expression on T cells appears to indicate memory cells, but CD8+ T cells down-regulate it after activation.

Area of Science:

  • Immunology
  • Veterinary Immunology
  • Cell Biology

Background:

  • CD45 isoforms play a critical role in T-cell activation and function.
  • Identifying specific CD45 isoforms in cattle can enhance understanding of bovine immune responses.
  • The CD45RO isoform is associated with memory T cells in various species.

Purpose of the Study:

  • To identify and characterize the CD45RO isoform in cattle using a novel monoclonal antibody (mAb) IL-A116.
  • To analyze the functional differences between CD45RO-expressing and non-expressing T-cell subsets in cattle.
  • To investigate the role of CD45RO expression in defining memory T cells in bovine peripheral blood mononuclear cells (PBMC).

Main Methods:

  • Development and application of a novel monoclonal antibody (mAb) IL-A116 for detecting the 180,000 MW CD45RO isoform in cattle.

Related Experiment Videos

  • Flow cytometry analysis to identify CD45RO+ and CD45RO- subsets within CD4+ and CD8+ T-cell populations.
  • Cell sorting experiments followed by functional assays (proliferation, cytokine transcript and protein analysis) and cytotoxic T-lymphocyte precursor assessment.

    • Main Results:

    • The CD45RO isoform was identified in cattle, with distinct expression patterns across immune cell types (CD4+ T cells, CD8+ T cells, gamma delta T cells, monocytes, granulocytes, B cells).
    • Within CD4+ T cells, CD45RO+ cells showed proliferative responses to antigen (ovalbumin) and produced multiple cytokines (IL-2, IL-4, IFN-gamma) upon stimulation, suggesting a memory phenotype.
    • While all gamma delta T cells were CD45RO+, antigenically primed CD8+ T cells down-regulated CD45RO expression after activation, indicating a dynamic regulation in this subset.

    Conclusions:

    • The novel mAb IL-A116 enables precise analysis of CD45 isoform expression and T-cell function in cattle.
    • CD45RO expression in bovine CD4+ T cells is a reliable marker for antigenically primed memory cells.
    • Antigenically primed CD8+ T cells down-regulate CD45RO expression post-activation, suggesting a distinct functional role or trafficking behavior compared to CD4+ T cells.