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Related Experiment Videos

Relationship between astrocytic processes and "perineuronal nets" in rat neocortex

I Blümcke1, P Eggli, M R Celio

  • 1Department of Histology and General Embryology, University of Fribourg, Switzerland.

Glia
|October 1, 1995
PubMed
Summary

Perineuronal nets (PNs) are not made of glial processes. Instead, these specialized extracellular structures are found between inhibitory neurons and astrocytes in the brain.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Histology

Background:

  • Perineuronal nets (PNs) are extracellular matrix structures that ensheath specific inhibitory interneurons in the mammalian neocortex.
  • A hypothesis suggested that PNs might be composed of glial cell processes.

Purpose of the Study:

  • To investigate the relationship between glial cells and perineuronal nets (PNs) in the rat neocortex.
  • To determine if PNs are formed by glial processes.

Main Methods:

  • Intracellular iontophoresis of Lucifer Yellow (LY) into glial cells in lightly fixed rat neocortical slices.
  • Visualization of PNs using Vicia villosa lectin.
  • Confocal laser scanning microscopy to analyze the spatial relationship between labeled glial processes and PNs.

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Main Results:

  • Glial processes and PNs were identified as distinct structures.
  • PNs were consistently located in the extracellular space between astrocyte processes and neurons.
  • Protoplasmic astrocytes, but not other glial cells, extended processes that contacted PNs.

Conclusions:

  • Perineuronal nets (PNs) are not composed of glial processes.
  • PNs are likely specialized extracellular material situated between inhibitory interneurons and surrounding astrocytic processes.