Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Idiotypic determinants on T-cell subpopulations

H Binz, H Frischknecht, F W Shen

    The Journal of Experimental Medicine
    |April 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Lyb-2 phenotype of hemolytic PFC.

    Immunogenetics·2012
    Same author

    Interlaboratory validation of oxidation-index measurement methods for UHMWPE after long-term shelf aging.

    Journal of biomedical materials research·2002
    Same author

    Interlaboratory studies to determine optimal analytical methods for measuring the oxidation index of UHMWPE.

    Biomaterials·2001
    Same author

    Interlaboratory reproducibility of standard accelerated aging methods for oxidation of UHMWPE.

    Biomaterials·2001
    Same author

    Stroke in hemoglobin (SD) sickle cell disease with moyamoya: successful hydroxyurea treatment after cerebrovascular bypass surgery.

    Blood·2001
    Same author

    Effect of sterilization method and other modifications on the wear resistance of acetabular cups made of ultra-high molecular weight polyethylene. A hip-simulator study.

    The Journal of bone and joint surgery. American volume·2000

    Killer T cells utilize distinct idiotypic receptors. Helper T cell idiotypes differ from killer T cell idiotypes, influencing immune responses and potentially enabling autoanti-idiotypic antibody therapies.

    Area of Science:

    • Immunology
    • Cellular immunology
    • T cell biology

    Background:

    • Killer T cells recognize foreign antigens via T cell receptors (TCRs) and major histocompatibility antigens.
    • Idiotypic receptors on T cells can be targeted by anti-idiotypic antibodies to modulate immune responses.
    • T cell subsets, such as Lyt-1+2-3- (helper) and Lyt-1-2+3+ (cytotoxic/killer), play distinct roles in immunity.

    Purpose of the Study:

    • To compare the idiotypic profiles of helper T cell blasts (Lyt-1+2-3-) and killer T cell blasts (Lyt-1-2+3+) generated in mixed leukocyte culture (MLC).
    • To investigate whether idiotypes on killer T cell precursors can induce effector functions.
    • To explore the potential of anti-idiotypic immunity to induce specific unresponsiveness.

    Main Methods:

    • Generation of T cell blasts (Lyt-1+2-3- and Lyt-1-2+3+) in mixed leukocyte culture (MLC) across an H-2 locus barrier.

    Related Experiment Videos

  • Absorption of anti-idiotypic antibodies with respective T cell blasts to assess idiotype specificity.
  • Inhibition assays to evaluate the effect of absorbed antiserum on MLC proliferation and killer T cell function.
  • Analysis of T cell blasts' capacity to induce specific unresponsiveness via anti-idiotypic immunity in syngeneic recipients.

    • Main Results:

    • Helper T cell blasts (Lyt-1+2-3-) and killer T cell blasts (Lyt-1-2+3+) display distinct idiotypes.
    • Idiotypes on killer T cell precursors can trigger the induction of effector T cell function.
    • Autoanti-idiotypic antibodies can bypass the requirement for helper T cells in generating allospecific killer T cells.

    Conclusions:

    • Helper T cells and killer T cells possess unique idiotypic receptors, differentiating their functions.
    • Killer T cell precursor idiotypes are crucial for initiating effector functions, suggesting a role in immune regulation.
    • Targeting T cell idiotypes with autoanti-idiotypic antibodies presents a potential therapeutic strategy for modulating immune responses, possibly circumventing the need for helper T cells.