GLC determination of methenamine in tablets

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Accurate quantification of methenamine in pharmaceutical formulations is crucial for quality control.
• Existing methods for methenamine determination may lack the desired speed or sensitivity.

Purpose of the Study:

• To develop and validate a rapid and sensitive gas-liquid chromatography (GLC) method for the quantitative determination of methenamine in tablet dosage forms.
• To compare the performance of the developed GLC method against the official National Formulary (NF) assay.

Main Methods:

• Quantitative analysis of methenamine using gas-liquid chromatography (GLC).
• Sample preparation involved tablet dissolution in ethanol with an internal standard (pentylenetetrazol).
• Chromatographic separation was performed on a stainless steel column (10% OV-17 on Chromosorb W-HP), with quantitation based on peak height measurements.

Main Results:

• The developed GLC method demonstrated high sensitivity and rapidity for methenamine quantification.
• The method proved to be accurate and reliable for analyzing methenamine in tablets.
• Peak height measurements provided effective quantitation.

Conclusions:

• The developed GLC method is a significant improvement over the official NF assay and other proposed methods.
• This method offers enhanced simplicity, directness, speed, and accuracy for methenamine determination in tablets.
• The validated GLC technique is suitable for routine pharmaceutical quality control.