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[Adenoviruses from stool samples in hospital units. Comparison with main pathogens in gastroenteritis (rotavirus,
N Durepaire1, M P Pradie, M C Ploy
1Service de Bactériologie-Virologie-Hygiène, CHU Dupuytren, Limoges, France.
Abstract:
During a six-years period (1988-1993), a total of 14,644 stool samples from in-patients of Limoges University Hospital were examined for the presence of principal enteric pathogens, such as adenovirus, rotavirus, Campylobacter, Salmonella, Shigella and others. Stools were processed for identification of bacteria by standard methods and viruses were detected in fecal specimens using antigen detection methods: ELISA (Enzyme Linked Immunosorbent Assay) and latex agglutination test. The decreasing rates of presence of enteric agents were respectively 6% for rotavirus, 3.2% for Salmonella, 2% for adenovirus, 1.6% for Campylobacter and 0.2% for Shigella, but according to the lack of sensibility of latex agglutination test, adenovirus prevalence was probably underestimated. Concerning the distribution of enteric pathogens throughout the year, our data demonstrate that rotavirus were rather shed during the months from January to April, adenovirus between April and August, Campylobacter during summer and Salmonella from July to October. The two thirds of Campylobacter and rotavirus infections and the half of adenovirus and Salmonella infections were identified during the ten first years of life. The highest prevalence occurs before 5 years old, during the 2nd year of life for adenovirus (4.4%) and rotavirus (22.3%) and during the 3rd year of life for Campylobacter (6.84%) and Salmonella (8.6%).
Insights
This study analyzed 14,644 stool samples to identify common enteric pathogens in hospital patients. Rotavirus and Salmonella were the most prevalent, with infections concentrated in children under ten years old.
Area of Science:
- Microbiology
- Infectious Diseases
- Public Health
Context:
- A six-year study (1988-1993) examined 14,644 inpatient stool samples at Limoges University Hospital.
- Focus on principal enteric pathogens including adenovirus, rotavirus, Campylobacter, Salmonella, and Shigella.
Purpose:
- To identify and quantify the prevalence of key enteric pathogens in a hospital inpatient population.
- To analyze the seasonal distribution and age-specific incidence of these infections.
Summary:
- Rotavirus (6%) and Salmonella (3.2%) showed the highest prevalence, while adenovirus (2%), Campylobacter (1.6%), and Shigella (0.2%) were less common.
- Seasonal patterns observed: Rotavirus (Jan-Apr), Adenovirus (Apr-Aug), Campylobacter (Summer), Salmonella (Jul-Oct).
- Infections predominantly affected children under ten, with peak prevalence in the second year for adenovirus and rotavirus, and third year for Campylobacter and Salmonella.
Impact:
- Highlights the significant burden of rotavirus and Salmonella infections in hospital settings.
- Provides crucial data for understanding enteric pathogen seasonality and age-specific risks.
- Informs public health strategies for prevention and control of pediatric gastrointestinal infections.