Occurrence of blastocystis sp. in cattle in Aragón, northeastern Spain

The occurrence of Blastocystis sp. in cattle is reported herein for the first time in Spain. Stool samples from 554 bovines ranging from 3 days old to adults collected from 30 cattle farms in Aragón (northeastern Spain) were concentrated by the formalin-ethyl acetate method and examined light microscopically for the presence of gastrointestinal parasites. Vacuolated cells of Blastocystis sp. was detected in fecal specimens from 10 calves (1.8%) aged from 7 weeks to 6 months. Infected calves were detected at 3 of the 30 farms surveyed (10%). Blastocystis sp. cells were rounded or oval, with a diameter ranging from 6.25 to 15 microns. Blastocystis infection in cattle was never correlated with diarrhea, although infection intensities were generally low.